纯度:98% by TLC;NMR (Conforms)
分子式:C24H32N4O5S2
分子量:520.66
溶剂:DMSO (30 mg/ml)
性状:Beige solid
存储:-20°C
Activity (short version):Precipitates RNA Binding Proteins
Function / Pharmacology:Exposure of cell or tissue lysates to biotin-isoxazole precipitates hundreds of RNA-binding proteins with significant overlap to the constituents of RNA granules.1,2 In one study roughly 580 nuclear proteins were identified as being precipitated by B-Isox including TAF15, RNA polymerase II (largest subunit), numerous subunits of the mediator complex as well as a variety of enzymes involved in epigenetic modification of histones.3 SR domains were found to be the determinant that facilitates B-Isox precipitation.4 Also allows for the fractionation / isolation of SG-associated proteins and RNA precipitates.5